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Andrisani,
Ourania M., Ph.D.
Professor of Molecular Biology
BA ‘77; MA ‘79; PhD ‘82; State University
of New York at Buffalo
E-mail: andrisao@purdue.edu
Research Interests
Regulation of eukaryotic gene expression;
protein-nucleic acid interactions; signal transduction; cell
growth control; neuronal cell differentiation. Current projects
address the mechanism of cellular transformation by viral
oncoproteins and signal transduction interplay in neuronal
cell differentiation. Recombinant DNA technology, e.g., cloning,
expression of foreign proteins in bacteria, site-directed
mutagenesis, in vitro protein-DNA interactions and gene transfer
in mammalian and avian cells are routine assays employed in
the laboratory.
Publications
Ji, M. and Andrisani,
O.M. (2005) The cAMP
signaling network represses sympathoadrenal cell development
and promotes melanogenesis in neural crest cells. Mol. Cell.
Biol., in press.
Bilodeau, M., Paris, M., Ji, M. and Andrisani,
O.M., (2005)
Adenosine signaling regulates differentiation of both neural
crest-derived and CNS-derived catecholaminergic neurons.
Mol. Cell. Neurons. in press.
W. H. Wang, G. Grégori, R. L. Hullinger, and O.
M. Andrisani, (2004) “Sustained Activation of p38 Mitogen-Activated
Protein Kinase and c-Jun N-Terminal Kinase Pathways by Hepatitus
B Virus X Protein Mediates Apoptosis via Induction of Fas/FasL
and Tumor Necrosis Factor (TNF) Receptor 1/TNF- Expression”,
Molecular And Cellular Biology 24:10352-65.
Johnston CA, Beazely MA, Bilodeau ML, Andrisani
O, Watts VJ. Differentiation-induced alterations
in cyclic AMP signaling in the Cath.a differentiated (CAD)
neuronal cell line. J Neurochem. 2004 Mar;88(6):1497-508.
Tarn C, Zou L, Hullinger RL, Andrisani
OM. Hepatitis B virus X protein activates the p38
mitogen-activated protein kinase pathway in dedifferentiated
hepatocytes.
J Virol. 2002 Oct;76(19):9763-72.
Lee S, Tarn C, Wang WH, Chen S, Hullinger
RL, Andrisani OM. Hepatitis B virus X protein
differentially regulates cell cycle progression in X-transforming
versus nontransforming hepatocyte (AML12) cell lines. J Biol
Chem. 2002 Mar 8;277(10):8730-40. Epub 2001 Dec 26.
Bilodeau ML, Greulich JD, Hullinger RL,
Bertolotto C, Ballotti R, Andrisani OM. BMP-2
stimulates tyrosinase gene expression and melanogenesis in
differentiated melanocytes. Pigment Cell Res. 2001 Oct;14(5):328-36..
Bilodeau, M.L., Boulineau, T., Greulich,
J.D.M., Hullinger, R.L. and Andrisani, O.M.
(2001) Differential expression of sympathoadrenal lineage-determining
genes and phenotypic markers in cultured primary neural crest
cells. In Vitro Cell. Dev. Biol., 37:185-192, 2001.
Tarn, C., S. Lee, Y. Hu, C. Ashendel and
O. Andrisani. (2001) Hepatitis B Virus protein
differentially activated ras-raf-MAPK and JNK pathways in
X-transforming versus non-transforming AML12 hepatocytes.
Jrn. Biol. Chem. In press (www.jbc.org)
Barnabas, S. and Andrisani, O.
(2000) Different regions of the hepatitis B virus X protein
are required for enhancement of bZip-mediated transactivation
vs. transrepression. J. Virol., 74, 83-90.
Bilodeau M., Boulineau, T., Hullinger, R.L.
and Andrisani, O. (2000) cAMP is a Dynamic
Modulator of Sympathoadrenal Cell Development in Neural Crest
Cell Cultures. Mol. Cell. Biol., 20(9):3004-3014.
Andrisani, O.M. and Barnabas,
S. (1999). The role of the transcriptional involvement of
HBV pX in hepatocarcinogenesis. (Review) International Journal
of Oncology, 15/2, pp. 373-379.
Andrisani, O.M., (1999).
Invited Review on CREB Mediated Transcriptional Control. Eds.
G.S. Stein, J.L. Stein, and J.B. Lian. Critical Reviews in
Eukaryotic Gene Expression, 9:19-32.
Tarn, C., Bilodeau, M., Hullinger, R., and
Andrisani, O., 1999. Differential immediate
early gene expression in conditional HBV px-transforming versus
non-transforming hepatocyte cell lines. J. Biol. Chem., Vol.
274:4, 2327-2336.
Barnabas S., Hai, T., and Andrisani,
O.M., 1997. The Hepatitis B Virus X Protein Enhances
the DNA-Binding Potential and Transcription Efficacy of bZip
Transcription Factors. J. Biol. Chem. Vol. 272:33, 20684-20690.
Williams, J.S. and Andrisani, O.M.,
1995. The hepatitis B virus X protein targets the basic region
leucine zipper domain of CREB. Proc. Natl. Acad. Sci. USA
92:3819-3823.
Andrisani, O.M. (1994)
Transcriptional involvement of the Hepatitis B virus X protein
in cellular transduction systems: Protein-Protein interactions
with bZip transactivators. In (Liver Carcinogenesis: The Molecular
pathways). Proceedings of the NATO Advanced Studies Institute,
Cell Biology Series.
Biol, C.J., Williams, J.S., Chou, C-H, Wang,
Q., Roach, P. and Andrisani, O.M., 1994.
The sequential phosphorylation at Ser129 in CREB341 is required
for the cAMP regulation of gene expression. J.Biol.Chem.,
1269, 32187-32193.
Williams, J.S., Dixon, J.E. and Andrisani,
O.M., 1993. Binding constant determination studies
utilizing recombinant ê CREB protein. DNA and Cell Biology,
12, 183-193.
Colbran, J.L., Fiol, C.J., Roach, P., Dixon,
J.E., Andrisani, O.M. and Corbin, J.D. (1993)
cAMP-dependent protein, but not the cGMP-dependent enzyme
rapidly phosphorylates DCREB and a synthetic DCREB peptide.
Bioch. and Cell Biol., 70, 1277-1282.
Santiago-Rivera, Z., Williams, J.S., Gorenstein,
D. and Andrisani, O.M., 1993. Bacterial expression
and characterization of the CREB bzip peptide. Circular Dichroism
and Initial 1H-NMR studies. Protein Science, 2, 1461-1471.
Andrisani, O.M. and Dixon,
J.E. (1991) Involvement of lysine residues 289 and 291 of
DCREB protein in the recognition of the cAMP response element.
J. Biol. Chem. 266, 21444-21450.
Andrisani, O.M. and Dixon,
J.E. (1990) Somatostatin Gene Regulation, Ann. Rev. Phys.
52, 793-806.
Andrisani, O. M. and Dixon,
J. E. (1990) Identification and Purification of a Novel 120
Kda Protein that Recognizes the cAMP Responsive Element, J.
Biol. Chem. 265, 3212-3218.
Andrisani, O. M., Zhu,
Z., Pot, D. A. and Dixon, J. E. (1989) In vitro transcription
directed from the somatostatin promoter is dependent upon
a purified 43-KDa DNA-binding protein. Proc. Natl. Acad. Sci.
U.S.A. 86, 2181-2185.
Zhu, Z., Andrisani, O. M.,
Pot, D. A. and Dixon, J. E. (1989) Purification and characterization
of a 43-KDa transcription factor required for rat somatostatin
gene expression. J. Biol. Chem. 264, 6550-6556.
Andrisani, O. M., Pot,
D. A., Zhu, Z. and Dixon, J. E. (1988) Three sequence specific
DNA-protein complexes are formed with the same promoter element
essential for expression of the rat somatostatin gene. Mol.
Cell. Biol. 8, 1947-1956.
Andrisani, O. M., Hayes,
T. E., Roos, B. and Dixon, J. E. (1987) Identification of
the promoter sequences involved in the cell specific expression
of the rat somatostatin gene. Nucl. Acids Res. 15, 5715-5728.
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