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Prasad,
Negendra, DVM, Ph.D.
Assistant Professor of Basic Medical Sciences
BVSc ‘88, University of Agricultural Sciences, Bangalore;
PhD ‘94, Memorial University of Newfoundland
E-mail:nprasad@purdue.edu
Research Interests
The primary focus of my research is phosphotidylinositol
signaling, and in particular, the role of 5’ inositol
phosphatase SHIP2 in Cancer. My previous work on SHIP2 in
tumor cells demonstrated a regulatory role for SHIP2 in cell
adhesion and spreading downstream of Src family kinases (Mol
Cell Biol. 2001, 21, 1416; J Cel Sci. 2002, 115, 3807). Based
on this and on preliminary results (unpublished) from RNA
interference (RNAi)-mediated gene silencing experiments in
tumor cells, I hypothesize that SHIP2 plays an important role
in tumor cell metastasis, survival and/or proliferation. An
elaborate study is underway to experimentally test this hypothesis
using multiple approaches including the generation of tissue-specific
genetic knockout mice.
Publications
N. K. Prasad and S. J. Decker,
April 1, 2005 “SH2-containing 5’ –Inositol
Phosphatas, SHIP2, Regulates Cytoskeleton of the Epidermal
Growth Factor Receptor” Journal of Biological Chemistry,
April 1, 2005:280:13129-36.
Prasad N, Topping RS and
Decker SJ (2002) Src Family Tyrosine Kinases Regulate Tyrosine
phosphorylation of 5'-Inositol Phosphatase SHIP2 during cell
attachment and spreading on Collagen I Journal of Cell Science,
115(Pt 19): 3807-3815
Prasad N, Topping RS and
Decker SJ (2001) SH2-Containing 5’ Inositol Phosphatase-2
(SHIP2) Associates With p130Cas Adapter Protein And Regulates
Adhesion And Spreading. Molecular and Cellular Biology 21
(4): 1416-1428
Prasad N, Topping RS, Zhou
D and Decker SJ (2000) Oxidative Stress and Vanadate Induce
Tyrosine Phosphorylation of Phosphoinositide-Dependent Kinase
1 (PDK1). Biochemistry, 39(23): 6929-6935
Sooryanarayana, Prasad N,
Bonnin E, Pashov A, Ben Jilani K, Ameisen JC, Kazatchkine
MD, and Kaveri SV (1999) Phosphorylation Of Bcl-2 And Mitochondrial
Changes Are Associated With Apoptosis Of Lymphoblastoid Cells
Induced By Normal Immunoglobulin G. Biochemical and Biophysical
Research Communications, 264(3): 896-901
Bar-Dayan Y, Sooryanarayana, Bonnin E, Prasad
N, Bar-Dayan Y, Kazatchkine MD and Kaveri SV (1999)
Apoptosis: The Relation Between Anti-Fas Antibodies And Immunosurveillance
Against Cancer And Autoimmunity. In: Cancer and Autoimmunity,
pp. Eds. Y. Shoenfeld, Elsever Science. (Invited book chapter)
Prasad NK, Papoff G, Zeuner
A, Bonnin E, Kazatchkine MD, Ruberti G and Kaveri SV (1998)
Therapeutic Preparations Of Normal Polyspecific Igg (I.V.Ig)
Induce Apoptosis In Human Lymphocytes And Monocytes: A Novel
Mechanism Of Action Of i.v.Ig Involving The Fas Apoptotic
Pathway. Journal of Immunology, 161(7): 3781-90.
Prasad KAN, Kazatchkine
M and Kaveri SV (1997) Mechanisms Of Action Of Ivig In Autoimmune
Disease. In: Pathogenic Autoimmune Reactions, pp. Eds. Paul,
S., The Humana Press Inc., Totowa, NJ, USA (Invited book chapter).
Kaveri S, Prasad N, Vassilev
T, Hurez V, Pashov A, Lacroix-Desmazes S and Kazatchkine M
(1997) Modulation Of Autoimmune Responses By Intravenous Immunoglobulin
(IVIg) Multiple Sclerosis, 3, 121-128 (Invited review)
Prasad KAN and Church JG
(1997) Characterization Of DNA Binding And Transcriptional
Regulatory Function Of An Endogenous Mutant P53 In MDA-468
Human Breast Cancer Cells. Biochemical and Biophysical Research
Communications, 232, 14-19
Prasad KAN and Church JG
(1997) EGF-Effects On P53 In MDA-468 Human Breast Cancer Cells:
Implications For G1 Arrest. Cell Proliferation, 30, 81-94
Prasad KAN and Church JG
(1991) EGF-Dependent Growth Inhibition In MDA-468 Human Breast
Cancer Cells Is Characterized By Late G1 Arrest And Altered
Gene Expression. Experimental Cell Research, 195, 20-26
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