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Robinson,
J. Paul, Ph.D.
Director, Purdue University Cytometry Laboratories, Professor
of Immunopharmacology, Professor of Biomedical Engineering
BS ‘75, New South Wales, Australia; MS ‘80, New
South Wales, Australia; PhD ‘83, New South Wales, Australia
E-mail:robinson@flowcyt.cyto.purdue.edu
Research Interests
Studies of immune function, especially phagocytic
cell function as related to the immune system. Our lab has
an interest in the role of oxygen and nitrogen radicals in
microvascular function, tissue injury, and wound healing.
Current projects are related to alterations in immune functions
after tissue injury, as well as studies on the effects of
toxic chemicals on immune function of neutrophils and monocytes.
We are also interested in application of flow cytometry to
identification of microbes using fluorescence and scatter
properties. Particular interests are in flow cytometry, confocal
microscopy, and development of both methodological and technological
advancements in this field. We have also developed expertise
in using multimedia tools for educational purposes. In this
area, our laboratory has been developing new technologies
for distribution of Internet based learning tools. In particular,
we have created material for high school biology using a WEB
based CD-ROMS.
Publications
Lu Zhou, Gerald Gregori, Jennier Masella
Blackman, J. Paul Robinson, Barry L. Wanner.
Stochastic activation of hte response regulator PhoB by noncognate
histidine
kinases. Journal of Inegrative Bioinformatics, 2005 in press
Garry L. Morefield, Anna Sokolovska, Dongping
Jiang, Harm HogenEsch, J.Paul Robinson, Stanley L. Hem, Role
of Aliminum-containing adjuvants in antigen internalization
by dendritic cells in vitro, Vaccine, 23 :1588-1595, 2005
J. Paul Robinson, Multispectral
Cytomety : The Next Generation. Biophotonics International
October 2004, 36-40.
Morefield, G.L. HogenEsch, H., Robinson,
J.P., Hem, S.L. Distribution of adsorbed antigen in mono-valent
and combination vaccines. Vaccine 22:1973-1984, 2004
Yinlong Sun, Bartek Rajwa and J.
Paul Robinson,
Adaptive Image-Processing Technique and Effective Visualization
of Confocal Microscopy Images Microscopy Research and Technique,
64:156-163, 2004
Bartek Rajwa, Helen A. Mcnally, Padma Varadharajan,
Jennifer Sturgis, J. Paul Robinson AFM/CLSM data visualization
and comparison using an open-source toolkit Microscopy Research
and Technique, 64:176-184, 2004
Yinlong Sun, Qiqi Wang, Haiying Xu, Bartek
Rajwa and J. Paul Robinson, Noise Reduction
and 3D Visualization of Confocal Microscopy Images, SPIE
Proceedings 5299 :63-
72 Computational Imaging II, Charles A. Bouman, Eric L. Miller,
Editors, 2004
Morefield GL, HogenEsch H, Robinson
JP, Hem SL. Related Articles, Links Distribution
of adsorbed antigen in mono-valent and combination vaccines.
Vaccine. 2004 May 7;22(15-16):1973-84
Huang TT, Geng T, Akin D, Chang WJ, Sturgis
J, Bashir R, Bhunia AK, Robinson JP, Ladisch
MR. Micro-assembly of functionalized particulate monolayer
on C18-derivatized SiO2 surfaces. Biotechnol Bioeng. 2003
Aug 20;83(4):416-27.
Wu J, Rajwa B, Filmer DL, Hoffmann CM, Yuan
B, Chiang C, Sturgis J, Robinson JP. Automated
quantification and reconstruction of collagen matrix from
3D confocal datasets. J Microsc. 2003 May;210(Pt 2):158-65.
Voytik-Harbin SL, Roeder BA, Sturgis JE,
Kokini K, Robinson JP. Simultaneous mechanical
loading and confocal reflection microscopy for three-dimensional
microbiomechanical analysis of biomaterials and tissue constructs.
Microsc Microanal. 2003 Feb;9(1):74-85.
Li N, Ragheb K, Lawler G, Sturgis J, Rajwa
B, Melendez JA, Robinson JP. DPI induces
mitochondrial superoxide-mediated apoptosis. Free Radic Biol
Med. 2003 Feb 15;34(4):465-77.
Huang TT, Sturgis J, Gomez R, Geng T, Bashir
R, Bhunia AK, Robinson JP, Ladisch MR. Composite
surface for blocking bacterial adsorption on protein biochips.
Biotechnol Bioeng. 2003 Mar 5;81(5):618-24.
Bassoe CF, Li N, Ragheb K, Lawler G, Sturgis
J, Robinson JP. Investigations of phagosomes,
mitochondria, and acidic granules in human neutrophils using
fluorescent probes. Cytometry. 2003 Jan;51B(1):21-9.
Li N, Ragheb K, Lawler G, Sturgis J, Rajwa
B, Melendez JA, Robinson JP. Mitochondrial
complex I inhibitor rotenone induces apoptosis through enhancing
mitochondrial reactive oxygen species production. J Biol Chem.
2003 Mar 7;278(10):8516-25. Epub 2002 Dec 20.
Luo J, Li N, Robinson JP,
Shi R. The increase of reactive oxygen species and their inhibition
in an isolated guinea pig spinal cord compression model.
Spinal Cord. 2002 Dec;40(12):656-65.
Roeder BA, Kokini K, Sturgis JE, Robinson
JP, Voytik-Harbin SL. Tensile mechanical properties
of three-dimensional type I collagen extracellular matrices
with varied microstructure. J Biomech Eng. 2002 Apr;124(2):214-22.
Brightman, A.O., Rajwa, B.P., Sturgis, J.E.,
McCallister, M.E., Robinson, J.P. and Voytik-Harbin,
S.L. Time-lapse confocal reflection microscopy of collagen
firbillogenesis and extracellular matrix assembly in vitro.
Biopolymers 54:222-234, 2000.
Gillespie, R.R., Hill, M.A., Kanitz, C.L.,
Knox, K.E., Clark, L.K., Robinson, J.P. Infection
of pigs by Aujeszky’s disease virus via the breath of
intranasally inoculated pigs. Res Vet Sci 68(3):217-222, 2000.
Robinson, J.P. Gravely,
B. White Light Scanning Digital Microscopy. In: Emerging Tools
for Single Cell Analysis: Advances in Optical Measurement
Technologies. J.P. Robinson and G.F.
Babcock (Eds.), pp. 291-306, New York: Wiley-Liss,
2000.
Robinson, J.P. Principles of Confocal Microscopy. In: Cytometry
3rd Edition, part A, Z.
Darzynkiewicz, H.A. Crissman, J.P.
Robinson. (eds), New York, Academic Press, 2000.
Robinson, J.P. and Sincock, S.A. Flow Cytometric Analysis
of Microorganisms. In: Cytometry 3rd Edition, Part B, Z. Darzynkiewicz,
H.A. Crissman, J.P. Robinson (eds) New York, Academic press,
2000.
Voytik-Harbin, S.L. 3D ECM substrates for
cell culture. In: Methods in Cell Biology: Cytometry, 3rd
edition. Z. Darzynkiewicz, H.A.Crissman, J.P. Robinson
(eds.) Academic Press, Sand Diego, CA. pp. 561-578, 2000.
Voytik-Harbin, S.L., Rajwa, B.P., Robinson,
J.P. 3D Imaging of ECM and ECM-Cell Interactions.
In: Methods in Cell Biology: Cytometry, 3rd edition. Z. Darzynkiewicz,
H.A. Crissman, J.P. Robinson (eds.) Academic Press, San Diego,
CA pp. 583-596, 2000.
Darzynkiewicz, Z., Robinson, J.P.,
and Crissman, H. (eds). Methods in Cell Biology, Cytometry,
part A, 3rd Edition (2000) Academic Press, New York. 29 Chapters,
Vol. 63.
Darzynkiewicz, Z., Robinson, J.P.,
and Crissman, H. (eds) Methods in Cell Biology, Cytometry,
Part B, 3rd Edition (2000) Academic Press, New York. 27 Chapters,
Vol. 64.
Shively, M.(Producer), Robinson,
J.P. (Publisher) May 2000, Cytometry CD-ROM Vol.
5.
Blanton, J.R., Jr., Grant, A.L., McFarland,
D.C., Robinson, J.P, and Bidwell, C.A. Isolation
of two populations of myoblasts from porcine skeletal muscle.
Muscle Nerve 22:43-50, 1999.
Robinson, J.P. Overview
of flow cytometry and microbiology. In Current Protocols in
Cytometry. Robinson, J.P., Darzynkiewicz, Z., Dean, P.N.,
Dressler, L., Orfao, A., Rabinovitch, P.S., Stewart, C.C.,
Tanke, H.J. and L.L. Wheeless, eds. New York, John Wiley,
1999.
Al-Majali, A.M., Robinson, J.P.,
Asem, E.K., Lamar, C., Freeman, M.J. and A.M. Saeed: Use of
flow cytometry to measure the interaction between Escherichia
coli heat-stable enterotoxin and its intestinal receptor in
mice. J.Immunol.Meth. 222:65-72, 1999.
Al-Majali, A., Asem, E., Lamar, C., Robinson,
J.P., Freeman, M.J. and M. Saeed. Effect of dietary
insulin on the response of suckling mice enterocytes to Escherichia
coli heat-stable enterotoxin. Vet.Res. 29:527-536, 1998.
Voytik-Harbin, S. L., Brightman, A. O.,
Waisner, B. Z., Robinson, J. P., and Lamar,
C. H. Small intestinal submucosa: A tissue-derived extracellular
matrix that promotes tissue-specific growth and differentiation
of cells in vitro. Tissue Eng. 4(2):157-174, 1998.
Narayanan, P.K. and Robinson, J.P.
The HL-60 cell: a cell culture model for studying phagocyte
cell functions. In Phagocyte Function: A Guide for Research
and Clinical
Evaluation. J.P. Robinson and G.F. Babcock, eds. New York,
Wiley-Liss, 1998.
Carter, W.O. and Robinson, J.P.
Neutrophil-endothelial cell interactions. In Phagocyte Function:
A Guide for Research and Clinical Evaluation. J.P. Robinson
and G.F. Babcock, eds. New York, Awiley-Liss, 1998.
Robinson, J.P., Oxygen
and nitrogen reactive metabolites and phagocytic cells. In
PhagocyteFunction: A Guide for Research and Clinical Evaluation.
J.P Robinson and G.F. Babcock, eds. New York, Wiley-Liss,
1998.
Phagocyte Function: A Guide for Research
and Clinical Evaluation. J.P. Robinson and
G.F. Babcock, eds. New York, Wiley-Liss, 1998.
Narayanan, P. K., Carter, W. O., Ganey,
P. E., Roth, R. A., Voytik-Harbin, S. L., and Robinson,
J. P. Impairment of human neutrophil oxidative burst
by polychlorinated biphenyls: inhibition of superoxide dismutase
activity. J. Leuk. Biol., 63: 216-224, 1998.
Electronic and Multimedia Publications:
10. Image & Microscopy, Microscopy
II, 5000 Copies ISBN 1-890473-07-3, August, 2004.
9. Cytometry CD-ROM Volume 8: May 2004
(Double CD-ROM), 5000 copies ISBN 1-890473-C6-5
8. Cytometry CD-ROM Vol. 7, Cytomics II,
Producer and Publisher: J. Paul Robinson, 2003. ISBN 0-9717498-8-4,
May 2003.
7. Cytometry CD-ROM Vol. 6, Cytomics, Producer
and Publisher: J. Paul Robinson, 2002. ISSN 1-890473-05-9,
May 2002.
6. Cytometry CD-ROM Vol. 5, Producer: Monica
Shively, Publisher: J. Paul Robinson, 1998. ISSN 1-890473-05-9,
May 2000.
5. Microscopy, Image Analysis and 3D Reconstruction - Volume
1, Producer: Monica Shively, Publisher, J. Paul Robinson,
1998, ISSN 1-890473-04-9.
4. Cytometry CD-ROM Volume 4 - ISAC Congress
Edition. Producer: Monica Shively, Publisher: J. Paul Robinson,
1998. ISSN 1-890473-03-9.
3. The Purdue CD ROM Vol. 3; 5000 copy
distribution published Aug 1997, producer Monica Shively,
publisher J. Paul Robinson. ISBN 1-890473-02-2
2. The Purdue Cytometry CD-ROM. Vol.
2. J. Paul Robinson, Purdue University. This production
includes a web based consolidation of educational material
in the field of cytometry, 10,000 files , 580 megabytes,
2500 distribution. (Dec, 1996) ISSN 1091-2037
1. The Purdue
Cytometry CD-ROM. Vol. 1. J. Paul Robinson, Producer & Director,
Purdue University. This production includes a web based
consolidation of educational material in the field of cytometry.
6,000 files , 520 megabytes, 1500 copy distribution. 1996.
(April, 1996)
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