Education

Overview of Research

Immunohistochemical characterization of neoplastic diseases. Development of diagnostic tests for infectious and neoplastic diseases using immunohistochemistry and other in situ techniques.

Publications

Refereed journals

1. Ramos JA, Camón J (1980) Coagulación intravascular diseminada (CID) en los animales domésticos. Disseminated intravascular coagulopathy (DIC) in domestic animals. Panorama Veterinario (May):197-214.

2. Ramos JA, Gómez-Lucía ME (1984) Mecanismos inmunológicos en la rinotraqueitis infecciosa bovina (IBR). Immunologic mechanisms in infectious bovine rhinotracheitis (IBR). Comunicaciones del INIA. Serie Higiene y Sanidad Animal. Number 7.

3. Cairó J, Ferrer L, Font J, Ramis A, Ramos J. (1984) Osteoartropatía hipertrófica asociada a una metástasis pulmonar de un carcinoma de mama. Hypertrophic osteoartropathy associated with pulmonary metastasis of a mammary carcinoma. Medicina Veterinaria 1:609-612.

4. Ferrer L, Domingo M, Fondevila D, Pumarola M, Ramis A, Ramos JA (1985) Diagnóstico histopatológico de las dermatitis del perro. Histopathological diagnosis of canine dermatitis. Medicina Veterinaria 2:681-689.

5. Domingo M, Ramos JA, Domínguez L, Ferrer L, Marco A (1986) Demonstration of Listeria monocytogenes with the PAP technique in formalin fixed and paraffin embedded tissues of experimentally infected mice. Journal of Veterinary Medicine B 33:537-542.

6. Ferrer L, Rabanal RM, Domingo M, Ramos JA, Fondevila D (1988) Identification of Leishmania donovani amastigotes in canine tissues by immunoperoxidase staining. Research in Veterinary Science 44:194-196.

7. Ramos JA, Domingo M, Domínguez L, Ferrer L, Marco A (1988) Immunohistologic diagnosis of avian listeriosis. Avian Pathology 17:227-233

8. Ferrer L, Rabanal R, Fondevila D, Ramos JA, Domingo M (1988) Skin lesions in canine leishmaniasis. Journal of Small Animal Practice 29:381-388.

9. Marco A, Ramos JA, Domínguez L, Domingo M, González L. (1988) Immunocytochemical detection of Listeria monocytogenes in tissue with the peroxidase-antiperoxidase technique. Veterinary Pathology 25:385-387.

10.Fondevila D, Ferrer L, Ramos JA, Montané V, Ramis AJ (1989) Immunohistochemical localization of S-100 protein and lysozyme in canine lymph nodes and lymphomas. Journal of Veterinary Medicine A 36:71-77.

11.Briones V, Dominguez L, Domingo M, Marco A, Ramos JA, García JA, Suárez G (1989) Serological diagnosis of listeriosis in man, sheep and rabbit by immunoperoxidase technique. Acta Microbiologica Hungarica 36:315-319.

12.Ramos JA, Ramis AJ, Rabanal RM, Marco A, Domingo M, Ferrer LM (1990) Scanning electron microscopy of swine lymphoid organs. Histology and Histopathology 5:397-406.

13.Ramos JA, Domingo M, Cabañes J, Marco A, Ramis AJ, Pumarola M (1990) Nocardiosis en un perro. Canine nocardiosis: a case report. Clínica Veterinaria de Pequeños Animales 10:157-162.

14.Ramis A, Ramos J, Fondevila D, Pumarola M, Ferrer L (1991) Estudio histoquímico e inmunohistoquímico del tejido linfoide porcino: ganglio linfático, bazo y timo. Histochemical and immunohistochemical study of swine lymphoid tissue: lymph node, spleen, and thymus. Anatomia, Histologia, Embryologia 20:154-168.

15.Ferrer L, Juanola B, Ramos JA, Ramis A (1991) Chronic colitis due to Leishmania infection in two dogs. Veterinary Pathology 28:342-343.

16.Ramos JA, Marco A, Kreeger JM, Altimira J, Domingo M (1991) Diagnóstico inmunocitoquímico de la leptospirosis canina. Immunocytochemical diagnosis of canine leptospirosis. Medicina Veterinaria 8:418-423.

17.Plana J, Vayreda M, Vilarrasa J, Bastons M, Rosell R, Martínez M, Pujols J, Badiola JI, Ramos JA, Domingo M, López E, San Gabriel A (1991) Síndrome reproductivo y respiratorio porcino (S.R.R.P.) o enfermedad misteriosa del cerdo (MPD): Resultados preliminares del aislamiento del agente causal y reproducción experimental de la enfermedad. Swine reproductive and respiratory syndrome (SRRP) or mystery swine disease (MSD): Preliminary results of the isolation of the causal agent and experimental reproduction of the disease. Medicina Veterinaria 8:588-595.

18.Miller MA, Ramos JA, Kreeger JM (1992) Cutaneous vascular neoplasia in 15 cats: clinical, morphologic, and immunohistochemical studies. Veterinary Pathology 29:329-336.

19.Ramos JA, Ramis AJ, Marco A, Domingo M, Rabanal R, Ferrer L (1992) Histochemical and immunohistochemical study of the mucosal lymphoid system in swine. American Journal of Veterinary Research 53:1418-1426.

20.Ferrer L, Ramos JA, Bonavia R, Cabañes J, Pumarola M (1992) Cryptococcosis in two cats seropositive for feline immunodeficiency virus. Veterinary Record 131:393-394.

21.Turk JR, Fales WH, Maddox C, Miller M, Pace L, Fischer J, Kreeger J, Johnson G, Turnquist S, Ramos JA, Gosser HS (1992) Pneumonia associated with Salmonella cholerae-suis infection in swine: 99 cases (1987-1990). Journal of the American Veterinary Medical Association 201:1615-1616.

22.Plana J, Vayreda M, Vilarrasa J, Bastons M, Rosell R, Martínez M, San Gabriel A, Pujols J, Badiola JI, Ramos JA, Domingo M (1992) Porcine epidemic abortion and respiratory syndrome (mystery pig disease). Isolation in Spain of the causative agent and experimental reproduction of the disease. Veterinary Microbiology 33:203-211.

23.Marco AJ, Prats N, Ramos JA, Briones V, Blanco M, Dominguez L, Domingo M (1992) A microbiological, histopathological and immunohistological study of the intragastric inoculation of Listeria monocytogenes in mice. Journal of Comparative Pathology 107:1-9.

24.Prats N, Briones V, Blanco MM, Altimira J, Ramos JA, Dominguez L, Marco A, (1992) Choroiditis and meningitis in experimental murine infection with Listeria monocytogenes. European Journal of Clinical Microbiology & Infectious Diseases 11:744-747.

25.Turk JR, Fales WH, Maddox CW, Ramos JA, Fischer JR, Johnson GC, Kreeger JM, Miller MA, Pace LW, Turnquist SE, Gosser HS (1993) Pleuropneumonia in Missouri swine. Journal of Veterinary Diagnostic Investigation 5:101-103.

26.Ramos-Vara JA, Ferrer L, Visa J (1994) Pathologic findings in a cat with cryptococcosis and feline immunodeficiency virus infection. Histology and Histopathology 9:305-308.

27.Ramos-Vara JA, Miller MA, López E, Prats N, Brevik L (1994) Reactivity of polyconal human CD3 antibody in lymphoid tissues of cattle, sheep, goat, rat and mouse. American Journal of Veterinary Research 55:63-66.

28.Ramos-Vara JA, Briones V, Segalés J, Vilafranca M, Sordé A, Miller M (1994) Concurrent infection with Streptococcus equisimilis and Leishmania in a dog. Journal of Veterinary Diagnostic Investigation 6:371-375.

29.Johnson GC, Miller MA, Ramos-Vara JA (1995) Comparison of argyrophilic nucleolar organizer regions (AgNORs) and mitotic index in distinguishing benign from malignant canine smooth muscle tumors and in separating inflammatory hyperplasia from neoplastic lesions of the urinary bladder mucosa. Journal of Veterinary Diagnostic Investigation 7:127-136.

30.Johnson GC, Fales WH, Maddox CW, Ramos-Vara JA (1995) Evaluation of laboratory tests for confirming the diagnosis of encephalitic listeriosis in ruminants. Journal of Veterinary Diagnostic Investigation 7:223-228.

31.Sallés CJ, Arús J, Giné J, Morán JF, Ramos-Vara JA (1995) Infecciones por herpesvirus en los primates. I. Herpesvirus neurotrópicos. Herpesvirus infections in primates. I. Neurotropic herpesvirus. Medicina Veterinaria 12:713-719.

32.Johnson GC, Maddox CW, Fales WH, Wolff WA, Randle RF, Ramos-Vara JA, Schwartz H, Heise KM, Baetz AL, Wesley IV, Wagner DE (1996) Epidemiologic evaluation of encephalitic listeriosis in goats. Journal of the American Veterinary Medical Association 208:1695-1699.

33.Ramos-Vara JA, Ortiz-Santiago B, Segalés J, Dunstan RW. (1996) Cutaneous leishmaniasis in two horses. Veterinary Pathology 33:731-734.

34.Ramos-Vara JA, Rook J, Scanlan CM, Mugli F, Yamini B. (1997) Fusobacterium necrophorum septicemia in a lamb: pathological and microbiological characterization. Journal of Veterinary Diagnostic Investigation 9:79-82.

35.Ramos-Vara JA, Smith EJ, Watson GL. (1997) Lymphosarcoma with plasmacytoid differentiation in a scarlet macaw (Ara macao). Avian Diseases 41:499-504.

36.Tengelsen LA, Yamini B, Mullaney TP, Bell TG, Render JA, Patterson JS, Steficek BA, Fitzgerald SD, Kennedy FA, Slanker MR, Ramos-Vara JA, (1997) A 12-year retrospective study of equine abortion in Michigan. Journal of Veterinary Diagnostic Investigation 9:303-306.

37.Juan-Sallés C, Ramos-Vara JA, Prats N, Solé-Nicolás J, Segalés J, Marco AL. (1997) Spontaneous herpes simplex in common marmosets (Callithrix jacchus). Journal of Veterinary Diagnostic Investigation 9:341-345.

38.Oliver Duran C, Ramos-Vara JA, Render JA. (1997) Porcine dermatitis and nephropathy syndrome: A new condition to include in the differential diagnosis list for skin discoloration in swine. Swine Health and Animal Production 5:241-244.

39.Ramos-Vara JA, Dubey JP, Watson GL, Winn-Elliot M, Patterson JS, Yamini B. (1997) Sarcocystosis in mink (Mustela vison). Journal of Parasitology 83:1198-1201.

40.Ramos-Vara JA, Lu J-J, da Silva AJ, Montone KT, Pieniazek NJ, Lee CH, Pérez L, Steficek BA, Dunstan RW, Craft D, Watson GL. (1998) Characterization of natural occurring Pneumocystis carinii pneumonia in pigs by histopathology, electron microscopy, in situ hybridization and PCR amplification. Histology and Histopathology. 13:129-136.

41.Ramos-Vara JA, Miller MA. (1998) Intestinal plasmacytoma in an African Hedgehog (Atelerix albiventris). Journal of Wildlife Diseases 34:377-380.

42.Juan Sallés C, Prats N, Marco AJ, Ramos-Vara JA, Borras D, Fernández J. (1998) Fatal acute toxoplasmosis in three golden lion tamarins (Leontopithecus rosalia). Journal of Zoo and Wildlife Medicine 29:55-60.

43.Ramos-Vara JA, Kopcha M, Richter E, Watson GL, Patterson JS, Juan-Sallés, Yamini B. (1998) Actinomycotic splenitis and intestinal volvulus in an alpaca (Lama pacos). Journal of Zoo and Wildlife Medicine 29:228-232.

44.Ramos-Vara JA, Duran O, Render JA, Patterson JS. (1998) Necrotising stomatitis (oral necrobacillosis) associated with Fusobacterium necrophorum in three sows. Veterinary Record 143:282-283.

45.Ramos-Vara JA, Miller MA, Pace LW, Linke RP, Common RS, Watson GL. (1998)Intestinal multinodular Aλ-amyloid deposition associated with extramedullary plasmacytoma in three dogs: clinicopathological studies and immunohistochemical identification. Journal of Comparative Pathology 119:229-239.

46.Ramos-Vara JA, Takahashi M, Miller MA, Pace LW, Craft D, Common R, Watson GL. (1998) Intestinal extramedullary plasmacytoma associated with amyloid deposition in three dogs: an ultrastructural and immunoelectron microscopic study. Ultrastructural Pathology 22:393-400.

47.Ramos-Vara JA, Segalés J, Oliver Duran C, Campbell K, Domingo M. (1999) Diagnosis of swine infectious diseases using immunohistochemistry. Swine Health and Production 7:85-91.

48.Segalés J, Ramos-Vara JA, Oliver Duran c, Porter A, Domingo M. (1999) Diagnosis of swine infectious diseases using in situ hybridization. Swine Health and Production 7:125-128.

49.Rosell C, Segalés J, M, Ramos-Vara JA, Folch JM, Rodriguez-Arrioja GM, Duran CO, Balasch M, Plana-Durán J, Domingo M (2000) Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome. Veterinary Record 146:40-43.

50.Ramos-Vara JA, Beissenherz ME (2000) Optimization of immunohistochemical methods using two different antigen retrieval methods on formalin-fixed, paraffin-embedded tissues: experience with 63 markers. Journal of Veterinary Diagnostic Investigation 12:307-311.

51.Kiupel M, Mueller PB, Ramos-Vara J, Irizarry A, Lin TL (2000) Multiple endocrine neoplasia in a dog. Journal of Comparative Pathology 123:218-221.

52.Ramos-Vara JA, Beissenherz ME, Miller MA, Johnson GC, Pace LW, Fard A, Kottler SJ (2000) Retrospective study of 338 canine oral melanomas with clinical, histologic, and immunohistochemical review of 129 cases. Veterinary Pathology 37:597-608.

53.Ramos-Vara JA, Miller MA, Preziosi D (2000) Glucagonoma in a jaguar (Panthera onca). Journal of of Zoo and Wildlife Medicine 31:563-565.

54.Fitzgerald SD, Taylor D, Ramos J, Cooley TM (2000) Clinical challenge- Oral mucosa, gingiva, lips: multipe papillomas (oral papillomatosis). Journal of Zoo and Wildlife Medicine 31:581-584

55.Marsh AE, Johnson PJ, Ramos-Vara J, Johnson GC (2001) Characterization of a Sarcocystis neurona isolate from a Missouri horse with equine protozoal myeloencephalitis Veterinary Parasitology 95:143-154.

56.Ramos-Vara JA, Beissenherz ME (2001) Cost comparison of immunohistochemical tests in a veterinary diagnostic laboratory: experience with 78 antibodies. Journal of Histotechnology 24:17-21.

57.Miller MA, Kottler SJ, Cohn LA, Johnson GC, Kreeger JM, Pace LW, Ramos-Vara JA, Turk JR, Turnquist SE (2001) Mammary duct ectasia in dogs: 51 cases (1992-1999). Journal of the American Veterinary Medical Association 218:1303-1307.

58.Ramos-Vara JA, Beissenherz ME, Miller MA, Johnson GC, Kreeger JM, Pace LW, Turk JR, Turnquist SE, Watson GL, Yamini B (2001) Immunoreactivity of A103, an antibody to Melan A, in canine steroid-producing tissues and their tumors. Journal of Veterinary Diagnostic Investigation 13:328-332.

59.Ramos-Vara JA (2001) Soft tissue sarcomas in the African hedgehog (Atelerix albiventris): microscopic and immunohistologic study of three cases. Journal of Veterinary Diagnostic Investigation 13:442-445.

60.Ramos-Vara JA, Miller MA, Johnson GC (2001) Immunohistochemical characterization of canine hyperplastic hepatic lesions and hepatocellular and biliary neoplasms with monoclonal antibody Hepatocyte Paraffin 1 (Hep Par 1) and a monoclonal antibody to cytokeratin 7. Veterinary Pathology 38:636-643.

61.Ramos-Vara JA, Miller MA, Johnson GC, Turnquist SE, Kreeger JM, Watson GL (2002) Melan A and S100 protein immunohistochemistry in feline melanomas: 48 cases. Veterinary Pathology 39:127-132.

62.Ramos-Vara JA, Miller MA (2002) Metastatic pulmonary adenocarcinoma in a llama (Lama glama). Journal of Veterinary Diagnostic Investigation 14:328- 331.

63.Bell TG, Butler KL, Sill HB, Stickle JE, Ramos-Vara JA, Dark MJ (2002) Autosomal recessive severe combined immunodeficiency of Jack Russell terriers. Journal of Veterinary Diagnostic Investigation 14:194-204.

64.Kleiboeker SB, Miller MA, Schommer SK, Ramos-Vara JA, Boucher M, Turnquist SE (2002) Detection and multigenic characterization of a herpesvirus associated with malignant catarrhal fever in white-tailed deer (Odocoileus virginianus) from Missouri. Journal of Clinical Microbiology 40:1311-1318.

65.Ramos-Vara JA, Miller MA, Johnson GC, Pace LW (2002) Immunohistochemical detection of thyroid transcription factor-1 (TTF-1), thyroglobulin, and calcitonin in normal, hyperplastic, and neoplastic thyroid gland. Veterinary Pathology 39:480-487.

66.Ramos-Vara JA, Franklin C, Miller MA (2002) Bronchitis and bronchiolitis in a cat with CAR bacillus-like organisms. Veterinary Pathology 39:501-504.

67.Juan-Sallés C, Marco A, Ramos-Vara JA, Resendes A, Vergés J, Valls X,Montesinos A (2002) Islet hyperplasia in callitrichids. Primates 43:179- 190.

68.Kuroki K, Kreeger JM, Cook JL, Tomlinson JL, Johnson GC, pace LW, Turnquist SE, Turk JR, Ramos JA, Miller MA (2002) Immunohistochemical analysis of matrix metalloproteinase-1, -3, and -13 in naturally occurring cartilaginous tumors of dogs. American Journal of Veterinary Research 63:1285-1291

69.Ramos-Vara JA, Kiupel M, Miller MA (2002) Diagnostic immunohistochemistry of infectious diseases in dogs and cats. Journal of Histotechnology 25:201-212

70.Ramos-Vara JA, Del Piero F, Kiupel M, Fitzgerald SD, Bermudez AJ, Johnson GC, Miller MA (2002) Diagnostic immunohistochemistry of equine and avian infectious diseases. Journal of Histotechnology 25:185-198

71.Ramos-Vara JA, Miller MA, Boucher M, Roudabush A, Johnson GC (2003) Immunohistochemical detection of uroplakin III, cytokeratin 7, and cytokeratin 20 in canine urothelial tumors. Veterinary Pathology 40:55-62.

72.Ramos-Vara JA, Ganjam I, Fales W (2003) Pulmonary necrobacillosis in a white-tailed deer. Veterinary Record 152:401-403.

73.Miller MA, Kottler SJ, Ramos-Vara JA, Johnson PJ, Ganjam VK, Evans TJ (2003) 3-Methylindole induces transient olfactory mucosal injury in ponies. Veterinary Pathology 40:363-370.

74.Miller MA, Ramos-Vara JA, Dickerson MF, Johnson GC, Pace LW, Kreeger JM, Turnquist SE, Turk JR (2003)Uterine neoplasia in thirteen cats. Journal of Veterinary Diagnostic Investigation 15:515-522.

75.Ramos-Vara JA, Miller MA (2003) Immunohistochemical characterization of canine epithelial and mesenchymal intestinal tumors with monoclonal antibody to Hepatocyte Paraffin 1 (Hep Par 1). The Histochemical Journal 34:397-401.

76.Buckweitz S, Kleiboeker S, Marioni K, Ramos-Vara J, Rottinghaus A, Schwabenton B, Johnson GC (2003) Serological, RT-PCR and immunohistochemical detection of West Nile virus in a clinically affected dog. Journal of Veterinary Diagnostic Investigation 15:324-329.

77.Ramos-Vara JA, Ojeda JL, Miller MA, Reid R, Craft D, Watson GL (2004) Glomerulocystic kidney disease in a Belgian Malinois dog. An ultrastructural, immunohistochemical and lectin-binding study. Ultrastructural Pathology 28:33-42.

78.Ramos-Vara JA; Loiacono CM; Williams III F; Pardo I; Lakritz J (2004) Pulmonary neoplasia in two llamas (Lama glama). Veterinary Pathology 41:520- 523.

79.Kottwitz JJ, Preziosi DE, Miller MA, Ramos-Vara JR, Maggs DJ, Bonagura JD (2004) Toxoplasmosis leading to heart failure in a Fennec fox (Fennecs zerda). Journal of the American Animal Hospital Association 40:501-507

80.Ramos-Vara JA, Kiupel M, Miller MA (2005) Veterinary diagnostic immunohistochemistry: a survey of 47 laboratories. Journal of Histotechnology 28:19-23.

81.Ramos-Vara JA, Miller MA, Johnson GC (2005) Immunohistochemical detection of thyroid transcription factor-1 (TTF-1) in canine pulmonary tumors. Veterinary Pathology 41:520-523.

82.Ramos-Vara JA (2005) Technical aspects of immunohistochemistry. Veterinary Pathology 42:409-430.

83.Vilches-Moure JG, Ramos-Vara JA (2005) Comparison of rabbit monoclonal and mouse monoclonal antibodies in immunohistochemistry in canine tissues. Journal of Veterinary Diagnostic Investigation 17:346-350.

84.Miller MA, Ramos-Vara JA, Kleiboeker SB, Larson RL (2005) Effect of delayed or prolonged fixation on immunohistochemical detection of bovine viral diarrhea virus type I in skin of two persistently infected calves. Journal of Veterinary Diagnostic Investigation 17:461-463.

85.Irizarry-Rovira A, Wolf A, Ramos-Vara JA (2006) Cutaneous melanophoroma in a green iguana (Iguana iguana). Veterinary Clinical Pathology 31:101-105.

86.Langohr IM, Ramos-Vara JA, Wu CC, Froderman SF (2006) Listeric meningoencephalomyelitis in a cougar (Felis concolor): characterization by histopathological, immunohistochemical, and molecular methods. Veterinary Pathology 43:381-383.

87.Miller MA, Aper RL, Fauber A, Blevins WE, Ramos-Vara JA (2006) Extraskeletal osteosarcoma associated with a retained surgical sponge in a dog. Journal of Veterinary Diagnostic Investigation 18:224-228.

88.Juan-Sallés C, Garner MM, Didier ES, Serrato S, Acevedo LD, Ramos-Vara JA, Bowers LC, Parás A (2006) Disseminated encephalitozoonosis in captive juvenile cotton-top (Saguinus oedipus) and neonatal emperor (Saguinus imperator) tamarins. Veterinary Pathology 43:438-446.

89.Hoenerhoff MJ, Janovitz E, Ramos-Vara J, Kiupel M (2006) Choroid plexus papilloma in a Scottish Highland cow. Journal of Comparative Patholology 135:146-149.

90.Owston MA, Wu CC, Ramos-Vara JA (2006) Hepatic yersiniosis in a cougar (Felis concolor). Journal of Veterinary Diagnostic Investigation 18:511-513.

91.Ramos-Vara JA, Miller MA (2006) Comparison of two polymer-based immunohistochemical detection systems: ENVISION+TM and ImmPRESSTM . Journal of Microscopy 224:135-139.

92.Maratea KA, Hooser SB, Ramos-Vara JA (2006) Degenerative myelopathy and vitamin A deficiency in a young black-maned lion (Panthera leo). Journal of Veterinary Diagnostic Investigation 18:608-611.

93.Ramos-Vara JA, Miller MA (2007)Immunohistochemical detection of protein gene product 9.5 (PGP 9.5) in canine epitheliotropic T-cell lymphoma (mycosis fungoides). Veterinary Pathology 44:74-79.

94.Mitsui I, Jackson LP, Couëtil LL, Lin TL, Ramos-Vara JA (2007) Hypertrichosis in a horse with alimentary T-cell lymphoma and pituitary involvement. Journal of Veterinary Diagnostic Investigation 19:128-132.

95.Miller MA, Hartnett SE, Ramos-Vara JA (2007), Interstitial cell tumor and sertoli cell tumor in the testis of a cat, Vet.Pathol. 44: 394-397

96.Maratea K, Ramos-Vara JA, Corriveau LA, Miller MA (2007) Interstitial cell tumor and gynecomastia in a rabbit. Veterinary Pathology (accepted January 2007).

97.Irizarry-Rovira A, Lennox A, Ramos-Vara JA Malignant melanoma in a zebra finch (Taeniopygia guttata): Cytologic, histologic, and ultrastructural characteristics. Veterinary Clinical Pathology (accepted Feb 2007).