CPB 69700 RESEARCH
SEMINAR
Huiling Wei, BVM, MSVPM
CPB Graduate Student in Virology
“Infection Of Cesarean-Derived Colostrum-Deprived Pigs With
Porcine
Circovirus Type 2 And Swine
Influenza Virus (H1N1)”
Thurs., March 12, 2009
VPTH 112
3:30 pm
.
ABSTRACT: Porcine circovirus
type 2 (PCV2) and swine influenza virus (SIV) are very important pathogens in
the porcine respiratory disease complex (PRDC), which is of economic
significance worldwide. The pathogenesis of PCV2/SIV co-infection is unknown.
In this study, we focused on establishing a challenge model for PCV2 to
determine if SIV influences PCV2 replication and causes more severe PCV2
associated disease. Twenty-four cesarean-derived colostrum-deprived
(CDCD) pigs were assigned randomly to 3 groups of 8 pigs each. Pigs were
inoculated sequentially with PCV2 and H1N1 SIV in group 1 at one week interval,
PCV2-1 only in group 2, and cell culture medium in group 3. Two pigs from each
group were necropsied at 12, 21, 28, and 35 days post
infection (DPI). There were no significant differences in the level of PCV2
genomic copies in serum, nasal swab, lymph node and lung between groups 1 and
2. The microscopic lesions associated with PCV2 in lymphoid tissues and lungs
were not significantly different between groups 1 and 2. The antibody titer to
PCV2 did not differ significantly between groups 1 and 2. In summary, pigs were
infected with PCV2 virus in both groups. The strain of influenza virus did not
influence PCV2 virus replication in dually infected animals.