CPB 69700 RESEARCH
SEMINAR
Sai Vikram Vemula, BS, MS
Graduate Student in Molecular Virology
Department of Comparative Pathobiology
“Construction of bovine adenoviral vectors using bacteriophage CRE/Lox P recombination system”
Thurs., April 2, 2009
VPTH 112
3:30 pm
.
Abstract: Clinical
utility of human adenoviral vectors is severely hampered due to the high
prevalence of vector neutralizing antibodies in human population and lack of
tissue specific targeting. Alternative vectors derived from nonhuman
adenoviruses are currently being developed to circumvent these limitations.
Bovine adenovirus-3 based vectors have demonstrated tremendous promise as
delivery vehicles in vaccination and gene therapy applications. Currently used
method for construction of recombinant bovine adenovirus-3 based vectors relies
on in vivo homologous recombination in bacteria followed by transfection
in to susceptible cells with full length genome clones. While the utility of
this approach is well established, its efficiency is very low and time
consuming. To address this problem, we intend to develop a strategy based on
the bacteriphage P1-derived CRE-LoxP
recombination system. Using this approach bovine adenoviral vectors could be
generated as a result of CRE-mediated site specific recombination between two
plasmids after their co-transfection in to FBRT- HE1
cell line expressing CRE recombinase.