Digital Histology Laboratory
LCME 502 - Human Histology and Cell Biology (Indiana University School of Medicine Lafayette-on the campus of Purdue University)
BMS 602- Histology for Biomedical Scientists
Slides were scanned at 40X magnification (unless specified otherwise)
STAINING REACTIONS
Silver stain – reticular fibers (Type III collagen)
Masson's Trichrome Stain (many variants) Helps to differentiate connective tissue (collagen-usually blue or green) from the surrounding cells (pink to red) and tissues such as muscle.
CULTURED FIBROBLASTS
fibroblasts (20X) Identify different stages of mitosis - prophase, metaphase, anaphase, telophase
EPITHELIUM - (Identify in the slides below)
simple squamous (lining of blood vessels (endothelium), lining of body cavities (peritoneum, pleura, pericardium: these outer layers referred to as mesothelium)
stratified squamous -keratinized - thick skin of epidermis or non keratinized (epidermis, oral cavity esophagus, vagina)
simple cuboidal (small ducts of exocrine glands, follicles of thyroid gland, many of the kidney tubules)
simple columnar (gall bladder, intestinal epithelial cells (enterocytes))
stratified cuboidal (large ducts of exocrine glands)
pseudostratified columnar (respiratory system (ciliated in portions), portions of reproductive system-vas deferens)
transitional epithelium (urinary system)
Exocrine glands
Identify glands with an apocrine, merocrine, and holocrine mechanism of secretion.
Identify and differentiate cells/glands containing serous and mucous secretions.
MUSCLE TISSUE (skeletal, cardiac, smooth)
diaphram - skeletal muscle IIdentfy muscle fiber bundles, muscle fibers, myfibrils, perimysium)
CONNECTIVE TISSUE - (LOOSE, DENSE, BONE, CARTILAGE)
growth plate -dog (Identify growth plate zones in hyaline cartilage -reserve, proliferation, hypertrophy, calcification, ossification; calcified cartilage, calcified trabecular bone, osteoid, osteoblasts, osteocytes, osteoclasts)
external ear-elastic cartilage (Identify elastic fibers. Note sebaceous glands in skin)
tendon - dense regular connective tissue (Identify fibrocytes)
NERVOUS TISSUE
ganglion adjacent to adrenal gland
sciatic nerve-human (Identify connective tissue layers (epineurium, perineurium, endoneurium; myleinated axons, schwann cell nuclei)
spinal cord-neuroglia stain (Identify differences between gray matter neuroglia and white matter neuroglia)
spinal cord-gold (Identify large motor neurons)
peripheral nerve-longitudinal section (Identify node of Ranvier, axon, Schwann cell nucleus, myelin sheath)
peripheral nerve-cross section-osmium fixation
INTEGUMENT
ceruminous gland -Masson (10X)
thick-skin (20X) Keratinized stratified squamous epithelium, sweat glands (stratified cuboidal in ducts), dense irregular connective tissue in dermis.
pacinian corpuscle (20X) Image taken from sample of thick skin just below the dermis in the hypodermis.
CIRCULATORY SYSTEM
heart-mouse-toluidine blue (100X) (Identify A-I bands in cardiac muscle fibers and intercalated discs)
heart-purkinje fiber (Identify large pale staining cells of Purkinje fiber (modified cardiac muscle cells)
heart-purkinje fiber-2 (pale cells beneath epicardium)
femoral artery and vein-rat (Elastin stain. Note the well defined internal elastic lamina in the artery. The artery also has an external elastic lamina. There is elastin tissue in the vein also, but not as organized as in the artery.
aorta-elastin stain (Identify dense regular elastic tissue in tunica media of aorta)
RESPIRATORY SYSTEM
lung-bronchiole and terminal bronchiole-40X
DIGESTIVE SYSTEM
tongue-circumvallate papillae (Identify taste buds in the papillae and salivary glands in the tongue (mucous or serous secretion?)
salivary gland -PAS-Alcian blue
esophagus-upper (Identify stratified squamous epithelium, muscularis mucosa?, submucosa, esophageal glands proper, muscularis externa of striated (skeletal) muscle)
esophagus-masson stain 20X (What level of the esophagus?)
esophagus-middle (Observe smooth and skeletal outer muscle layers)
esophagus-stomach (cardiac) junction
esophagus-stomach (cardiac) junction-PAS stain
stomach-pylorus (Identify branched, mucous secreting glands)
stomach-fundus (Identify parietal cells, chief cells, mucous neck cells, enteroendocrine cells, surface mucous cells, gastric pits)
duodenum (Masson stain) (Identify mucosa, submucosa, Brunner's glands, muscularis mucosa, muscularis externa, myenteric plexus, lacteals, lamina propria)
jejunum-pig (Identify mitotic figures and Paneth cells in crypts, muscularis mucosa, Meissner's plexus, submucosa, muscularis externa)
appendix - human (Identify lymphoid nodules, lymphocytic infiltration of epithelium. How would you differentiate this section from ileum?)
LIVER & PANCREAS
liver-one micron thick plastic section-toluidine blue
liver-1 micron plastic section-2-toluidine blue
liver-PAS (Identify portal area with bile duct, hepatic artery, and portal vein, central vein, hepatocytes, Kuppfer cells, endothelial cells. What does the PAS staining around the central vein region represent? What is the significance for the function of the liver?)
gall bladder-20X (A portion of the gall bladder scanned at 40X - gall bladder-40X)
pancreas-H&E (zymogen granules in acinar cells -acidophilic; basophilic staining in cytoplasm of acinar cells-rough endoplasmic reticulum)
pancreas-Masson stain (variation)
ENDOCRINE SYSTEM
adrenal gland (Identify cortical layers, adipose tissue, muscular arteries, capsule)
adrenal gland - mouse (Identify outer cortex consisting of zona glomerulosa (mineralocorticoids), zona fasciculata (glucocorticoids), zona reticularis (gonadocorticoids) and inner medulla (cells make epinephrine and norepinephrine)
pituitary gland (Identify pars distalis, pars intermedia, pars nervosa, chromophiles (acidphils, basophils) chromophobes, pituicytes, Herring bodies)
BLOOD
basophil-human-Wright's stain (Bilobed nucleas frequently obscured by the basophil granules)
eosinophil-human (Bilobed nucleus, pink (eosinophilic) staining granules)
neutrophil-human (Trilobed nucleus most common)
FEMALE REPRODUCTIVE SYSTEM
oviduct-human (Identify peg cells in oviduct epithelium)
placenta-human (20X)
MALE REPRODUCTIVE SYSTEM
testis (In the seminiferous tubules-identify: Sertoli cells, spermatogonia, type I, type II spermatocytes, spermatids. In the tunica propria identify Leydig cells)
prostate-human-old Corpora amylacia present
penile urethra 20X Glands of Littre; corpus spongiosum surrounding urethra
penis corpus cavernosum Note helicine arteries; tunica albuginea on periphery
vas deferens-pampiniform plexus
URINARY SYSTEM
urinary-bladder-Masson stain, 20X
ureter-human (Identify transitional epithelium;
kidney (gold) (Identify glomerulus, macula densa, Bowman's capsule, urinary space, mesangial cells, podocytes, proximal convoluted tubules, distal convoluted tubules, medullary rays, vasa recta)
kidney (PAS stain) (Identify basement membane)
kidney H&E stain. Note transitional epithelium of renal calyx.
kidney (rat, medulla, H&E stain)
kidney (human, cortex-medulla junction, H&E, 20X)
LYMPHOID SYSTEM
spleen-mouse (Identify capsule, red pulp, white pulp, central artery, sinusoids)
spleen -PAS stain (Note discontinuous endothelium around splenic sinuses)
appendix - human (Identify lymphoid nodules, lymphocytic infiltration of epithelium. How would you differentiate this section from ileum?)
lymph node (Identify cortex, medulla, medullary sinuses, germinal center in lymphoid nodule)
lymph node-silver stain (Identify reticular connective tissue)
thymus-human-infant (Identify cortex, medulla, Hassal's corpuscles)