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PVM Directory | Intranet


Dear Internaut,

Please note that on the "Special Stains and Other Services" tab, those are average prices. If you request any of those, it will be broken down in tech time and supplies.

Paraffin Processing

Purdue Customers

Test #

Item

Cost

/unit

1

Processing and Embedding

$ 7.00

 /Cassette

2

Routine microtomy, 1st H&E, coverslip 

$ 6.00

 / Block

3

Additional H&E slides #

$ 5.50

 / slide

4

Routine microtomy, 1st unstained

$ 5.75

 / block

5

Additional Unstained slides #

$ 3.97 

/ slide

External Customers

Test #

Item

Cost

per unit

1

Processing and Embedding

$ 9.29

/ Cassette

2

Routine microtomy, 1st H&E, coverslip 

$ 8.72

/ Block

3

Additional H&E slides #

$ 6.97

/ slide

4

Routine microtomy, 1st unstained

$ 5.75 

/ block

5

Additional Unstained slides #

$ 3.97

/ slide

*External customers, please note: F&A costs are not included. As of November 2012, F&A costs were around 52%

Plastic Processing

Purdue Customers

Test #

Item

Cost

per unit

6

Plastic - large ground section + 1 T. Blue

$ 212.67

/ Specimen

7

Plastic -small ground section + 1 T.Blue

$ 88.43

/ Specimen

8

Plastic- microtome + 1 H&E

$ 46.52

/ Specimen

9

Additional Large plastic slide  (ground section)

$ 134.36

/ block

10

Additional small plastic slide (ground section)

$ 68.25

/ slide

11

Additional PMMA slide (thin section – microtome)

$12.78

/ slide

External Customers

External customers, please note: F&A costs are not included. As of November 2012, F&A costs were around 52%

Test #

Item

Cost

per unit

6

Plastic - large ground section + 1 T. Blue

$ 212.67

/ Specimen

7

Plastic -small ground section + 1 T.Blue

$ 88.43

/ Specimen

8

Plastic- microtome + 1 H&E

$ 46.52

/ Specimen

9

Additional Large plastic slide  (ground section)

$ 134.36

/ block

10

Additional small plastic slide (ground section)

$ 68.25

/ slide

11

Additional PMMA slide (thin section – microtome)

$12.78

/ slide

Special Stains

Class 1 Special Stain

Please note that the price above mentioned is an estimate. Depending on how many slides need to be stained, the prices may change and are based on tech time and supplies used.

Alcian Blue – demonstrates acid mucopolysaccharides

Masson’s Trichrome – differentiate between collagen & smooth muscle

Verhoeff’s - elastic fibers
$11/slide

Oil Red O – fat – frozen tissue only

Toluidine Blue – mast cells
$6/slide

Prussian Blue – ferric iron
Less than 20 slides - $14 per slide plus 0.5 hr of labor
20 slides or more -   $11 per slide plus 0.5 hr of labor

Saffranin O - Fast Green – Cartilage, glicosaminoglycans (GAGs)
$6/slide

Picrosirius Red
$10.50 per slide

PTAH muscle, collagen, fibrin
<20 slides  $14 per slide plus 1.0 hr of labor
  >20 slides  $11 per slide plus 1.0 hr of labor

ADDL stains Gram stain for bacteria (we have no controls) $10
 PAS (cost of Schiffs reagent) $10
 Gomoris silver stain $10

Class 2 Special Stain

$15.50 per slide

Von Kossa’s – calcium

Immuno Histochemistry - IHC

The prices for IHC vary a lot depending on the size of the tissue and time *wisely* spent validating the protocol.
Our prices ranged from $15 to $25 per slide, including validation.

Please contact Dr. Paul Snyder or Carol Bain if you are interested having us performing IHC for you.
Here are some examples of sucessful IHC protocols performed bu us.

On Mouse tissue

VCAM
CD 45 - lymphoid cells
alpha-SMA - aplha smooth muscle actin
MAC3 - macrophages
F4-80 - macrophages
CD3 - T-cells
Ki 67 - proliferation marker
UCP-1 - mitochondria
TH - Tyrosine Hydroxilase - Dopamine neurons

On Dog tissues
Nag-1
Cox-2