Bovine Respiratory PCR Screen (Viral with M. bovis)
Section: Molecular Diagnostics
Fee: $55.00
Turnaround Time: 1 – 2 business days
Days Test Started: Wednesday
Species: Bovine
Specimen
Name
Container
Minimum Amount
Lung
Sealed,Leakproof container
8-10 grams
Deep nasal or nasopharyngeal swab
Viral Transport Medium/Leakproof Tube
Tracheobronchial lavage fluid
Viral Transport Medium/Leakproof Tube
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date. LUNG: Several samples of the lesioned lung.
SWABS: Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile saline/PBS. Any bacterial swab system (Amies agar, with or without charcoal) is unacceptable and will not be tested.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
Additional Information
Test includes Bovine Respiratory Syncytial Virus (BRSV) PCR, Parainfluenza 3 Virus PCR (PI3), Bovine Coronavirus (BCV) PCR , Bovine Viral Diarrhea Virus (BVD) PCR , and Bovine Herpesvirus 1 (IBR) PCR .Swabs from the nares or cranial nasal cavity have limited ability to identify BRSV infection.
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