Porcine Circovirus 2/3 (PCV2 and PCV3) Duplex PCR
Section: Molecular Diagnostics
Fee: $44.00
Turnaround Time: 1 – 2 business days
Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday
Species: Porcine
Specimen
Name
Container
Minimum Amount
Serum
Red top vacutainer tube
1-2 ml
Oral Fluid (no pooling)
Sealed, Leakproof Plastic Conical Tube
5 - 10 ml
Lung
Sealed, Leakproof Container
Several samples
Lymph Node
Sealed, Leakproof Container
Several samples
Lung/lymph node pool
Sealed, Leakproof Container
Several samples each
Kidney
Sealed, Leakproof Container
Several samples
Processing Fluid
Sealed, Leakproof Container
5 - 10 ml
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
POOLING - Collect, identify, and submit samples individually. The laboratory will pool samples. SERUM - Collect blood in a red top or tiger top blood tube. Allow the sample to clot at room temperature before centrifuging or refrigerating. Serum may be submitted in a spun-down tiger top or poured off after spinning into a secondary tube. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on the submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time.
ORAL FLUID - Submit oral fluid in a leakproof container (i.e. plastic conical tube) with appropriate labeling.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
Additional Information
This test tests detects both PCV2 and PCV3. The report will contain results for both PCV2 and PCV3.Sequencing of ORF 2 is available for PCV2 with Porcine Circovirus Type 2 (PCV2) ORF 2 Sequencing
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