Bovine Coronavirus PCR (BCV)
Section: Molecular Diagnostics
Fee: $38.50
Turnaround Time: 1–2 business days
Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday
Species: Bovine
Specimen
Name
Container
Minimum Amount
Nasal/Pharyngeal Swab
Viral Transport Medium/Leakproof Tube
Lung
Leakproof container
Several specimens of lesioned lung
Feces
Leakproof container
5-10 gms
Fecal swab
Viral Transport Medium/Leakproof Tube
Colon/Ileum
Leakproof Container
2-3 segments
Intestine
Sealed Container or Whirlpak
2-3 segments
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
FECES - Collect feces in a leak proof container (sealable plastic bag or jar - do NOT submit in gloves). Collect feces that is free of debris and bedding.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leak proof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leak proof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FECES - Ensure container is securely sealed. Double bag all diarrhea samples to contain any leaks.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
Additional Information
This assay will detect both respiratory and enteric forms of bovine coronavirus.This test is also available as part of the Bovine Respiratory Virus Screen PCR . (BRSV, IBR, PI3, BCV, BVD).
This test is also part of the Bovine Neonatal Diarrhea Screen PCR (Bovine Coronavirus, Bovine Rotavirus, Crypto, Salmonella, and F5/K99).
This test is also part of the Bovine Coronavirus and Bovine Rotavirus Duplex PCR .
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