College of Veterinary Medicine

Canine Distemper (CDV) Virus Isolation

Section: Virology

Fee: $44.00

Turnaround Time: 2–3 weeks

Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday

Species: Canine

Specimen

Name

Container

Minimum Amount

Brain, Tonsil, Spleen, Lung, Liver, Bladder

Sealed Leakproof Container or Whirlpak

1-10g



Oral Mucosa

Viral Transport Medium/Leakproof Tube

1-10g


Whole Blood

Purple Top Vacutainer (EDTA)

5-10 ml


Serum

Red Top Vacutainer

1-2 ml


Swab

Viral Transport Medium/ Leakproof Tube


Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, and Date.
REFRIGERATION - Refrigerate all samples until shipment.
SERUM - Collect blood in a red top or tiger top blood tube. Allow the sample to clot at room temperature before centrifuging or refrigerating. Serum may be submitted in a spun-down tiger top or poured off after spinning into a secondary tube. If submitting specimens from multiple animals, 1) label each tube sequentially (e.g., 1, 2, 3…) to correspond to the specimen/animal order on the submission form; 2) for each specimen, list animal IDs on the submission form; and, 3) if >10 animals per submission, please submit an electronic spreadsheet with animal IDs (in sequential order) – this will facilitate accessioning and data entry, reducing turn-around time.
EDTA BLOOD - Collect blood in an appropriate vacutainer tube, making sure to meet minimum quantity.
FECES - Collect feces in a leakproof container (sealable plastic bag or jar - do NOT submit in gloves). Collect feces that is free of debris and bedding.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label them with tissue type, animal ID, and owner name.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture). DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
SERUM - Package blood tubes in a blood tube box or wrap them securely with a paper towel or bubble wrap to provide protection.
EDTA BLOOD - Package blood tubes in a blood tube box or wrap them securely with a paper towel or bubble wrap to provide protection.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.

Additional Information
Virus isolation of Canine distemper.

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