Bovine Respiratory Syncytial Virus (BRSV) PCR
Section: Molecular Diagnostics
Fee: $38.50
Turnaround Time: 2–8 business days
Days Test Started: Wednesday, Friday
Species: Bovine, Caprine, Ovine, Ruminant
Specimen
Name
Container
Minimum Amount
Lung
Sealed, Leakproof Container or WhirlPack
8 - 10 grams
Deep Nasal or Nasopharyngeal Swab
Viral Transport Medium/Leakproof Tube
Tracheobronchial lavage
Viral Transport Medium/Leakproof Tube
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name. SWABS: Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile saline/PBS. Any bacterial swab system (Amies agar, with or without charcoal) is unacceptable and will not be tested.
LUNG: Several samples of lesioned lung.
Refrigerate all samples until shipment.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
Additional Information
Swabs from the nares or cranial nasal cavity have limited ability to identify BRSV infection.Also available as part of the Bovine Respiratory Virus Screen PCR (BRSV, IBR, PI3, BCV, BVD)
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