Ranavirus PCR

Section: Molecular Diagnostics

Fee: $33.00

Turnaround Time: 1–2 business days

Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday

Species: Fish, Other

Specimen

Name Container Minimum Amount
Skin SwabLeakproof Container
Oral SwabLeakproof Container
Ventral SwabLeakproof Container
Cloacal SwabLeakproof Container
Environmental SwabLeakproof Container
LiverLeakproof Container or Whirlpak5 grams
KidneyLeakproof Container or Whirlpak5 grams
SpleenLeakproof Container or Whirlpak5 grams
Whole Blood (EDTA)Purple Top Vacutainer1 mL
Cell Culture IsolateLeakproof Container or Tube

Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
EDTA BLOOD - Collect blood in appropriate vacutainer tube making sure to meet minimum quantity.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leak-proof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture). DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leak-proof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double-bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
EDTA BLOOD - Package blood tubes in a blood tube box or wrap securely with a paper towel or bubble wrap to provide protection.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double-bag ALL fresh tissues. LEAKS - Ensure all samples are in leak-proof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double-bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.