Leptospira spp. PCR

Specimen

Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
EDTA BLOOD - Collect blood in appropriate vacutainer tube making sure to meet minimum quantity.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
FETUS/FETAL TISSUES - Refrigerate and store the body until it is able to be delivered to the lab for necropsy. Deliver to ADDL for necropsy as soon as possible to minimize autolysis. Remember to collect and submit placenta (cotyledonary NOT amniotic).

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.

Additional Information

This tests detects pathogenic Leptospira spp. at the genus level. Results reported as positive or negative.
**If less than the ideal amount of urine is received, the test will be completed, but there will be a note with the test result stating that less than the required amount of sample was used to complete this test.
This test is also part of the ruminant (bovine, ovine, caprine) abortion panels - see Ruminant Abortion LTN Multiplex PCR (Leptospira, Toxoplasma, and Neospora).