Salmonella spp. PCR
Section: Molecular Diagnostics
Fee: $38.50
Turnaround Time: 2 – 3 business days
Days Test Started: Wednesday, Friday
Species: All Species
Specimen
Name
Container
Minimum Amount
Feces
Sealed, Leakproof Container
8 - 10 grams
Fecal swab
Viral Transport Medium/Leakproof Tube
Intestines
Sealed, Leakproof Container
2-3 segments
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
FECES - Collect feces in a leakproof container (sealable plastic bag or jar - do NOT submit in gloves). Collect feces that is free of debris and bedding.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FECES - Ensure the container is securely sealed. Double bag all diarrhea samples to contain any leaks.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
Additional Information
This test detects Salmonella spp. at the genus level. Reported as positive or negative. If serogroup identification is required, Salmonella culture should be requested.This test is also available as part of the Porcine Enteric Pathogen Panel PCR (Salmonella, Lawsonia, Brachyspira).
This test is also part of the Bovine Neonatal Diarrhea Screen PCR (Bovine Coronavirus, Bovine Rotavirus, Crypto, Salmonella, and F5/K99).
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