College of Veterinary Medicine

Brucella spp. PCR

Section: Molecular Diagnostics

Fee: $38.50

Turnaround Time: 1 – 2 business days

Days Test Started: Wednesday, Friday

Species: Bovine, Canine, Caprine, Equine, Ovine, Porcine, Ruminant

Specimen

Name

Container

Minimum Amount

Swabs

Leakproof Tube with 0.5ml sterile saline/PBS


Liver

Leakproof Container

8 - 10 grams


Placenta (cotyledons)

Leakproof Container

8 - 10 grams


Fetal Tissues

Leakproof Container

8 - 10 grams


Milk

Leakproof Container

5-10 ml


Whole blood

EDTA (purple) vacutainer

3-4 ml


Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date. SWABS for PCR: Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a commercial transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile saline/PBS. Refrigerate all samples until shipment.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leak proof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues. LEAKS - Ensure all samples are in leak proof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu. OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.

Additional Information
Detects Brucella spp. at the genus level.
This test is also part of the ruminant (bovine, ovine, caprine) abortion panels - see Ruminant Abortion C3B Multiplex PCR (Chlamydia and Brucella).

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