Feline Coronavirus (FCoV) PCR
Section: Molecular Diagnostics
Fee: $38.50
Turnaround Time: 1–2 business days
Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday
Species: Feline
Specimen
Name
Container
Minimum Amount
Lung, Liver, Spleen, Kidney, Instestine
Sealed, Leakproof Container
5 grams
Whole Blood
Purple Top Vacutainer (EDTA)
2-5 ml
Fetal Tissues
Sealed, Leakproof Container
5 grams
Swab-Ocular, Nasal
Sterile Vial with Viral Transport Media
BAL
Sealed, Leakproof Conainter
1-2ml
Feces
Sealed, Leakproof Container
2-5 grams
Peritoneal, Pleural Fluid
Sealed, Leakproof Container
1-2 ml
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, and Date.
REFRIGERATION - Refrigerate all samples until shipment.
EDTA BLOOD - Collect blood in an appropriate vacutainer tube, making sure to meet minimum quantity.
FECES - Collect feces in a leakproof container (sealable plastic bag or jar - do NOT submit in gloves). Collect feces that is free of debris and bedding.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label them with tissue type, animal ID, and owner name.
FETUS/FETAL TISSUES - Refrigerate and store the body until it can be delivered to the lab for necropsy. Deliver to ADDL for necropsy as soon as possible to minimize autolysis. Remember to collect and submit the placenta (cotyledonary, NOT amniotic).
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
EDTA BLOOD - Package blood tubes in a blood tube box or wrap them securely with a paper towel or bubble wrap to provide protection.
FECES - Ensure the container is securely sealed. Double bag all diarrhea samples to contain any leaks.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with the owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
FETUS/FETAL TISSUES - Double bag body if shipping through a courier service. Ship the placenta in a separate leakproof plastic bag.
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