Brachyspira hyodysenteriae PCR
Section: Molecular Diagnostics
Fee: $38.50
Turnaround Time: 1–2 business days
Days Test Started: Wednesday, Friday
Species: Porcine
Specimen
Name
Container
Minimum Amount
Feces
Sealed, Leakproof Container
10 - 20 grams
Colon content
Sealed, Leakproof Container
10-20 grams
Colon
Sealed, Leakproof Container
Several segments, 2-3 inches long
Fecal swab
Sealed, Leakproof container
Collection Information
GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
FECES - Collect feces in a leakproof container (sealable plastic bag or jar - do NOT submit in gloves). Collect feces that are free of debris and bedding.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label them with tissue type, animal ID, and owner name.
Shipping Information
OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture). DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FECES - Ensure the container is securely sealed. Double bag all diarrhea samples to contain any leaks.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
Additional Information
Also available as part of the Porcine Enteric Pathogen Panel PCR (Salmonella, Lawsonia, Brachyspira).- Indiana Animal Disease Diagnostic Laboratory Home
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