Porcine Enteric Coronavirus Multiplex PCR Screen (PEDV, PDCoV, TGE)

Section: Molecular Diagnostics

Fee: $38.50

Turnaround Time: 1–2 business days

Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday

Species: Porcine

Specimen

Name Container Minimum Amount
FecesLeakproof Container5 - 10 grams
Oral FluidSealed Tube5-8 ml
IntestineLeakproof container or WhirlPack2-3 segments
Rectal/Fecal SwabViral Transport Medium or Saline
Processing FluidSealed Tube5-8 ml

Collection Information

GENERAL - Label all containers with appropriate information - Animal ID, Owner Name, Veterinarian, Sample Type, Date.
REFRIGERATION - Refrigerate all samples until shipment.
FECES - Collect feces in a leak proof container (sealable plastic bag or jar - do NOT submit in gloves). Collect feces that is free of debris and bedding.
SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leak proof tube with 0.5 ml sterile. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
ORAL FLUID - Submit oral fluid in a leak proof container (i.e. plastic conical tube) with appropriate labeling.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easily matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
FECES - Ensure container is securely sealed. Double bag all diarrhea samples to contain any leaks.
SWAB - Ship swabs in a sealed container with the appropriate media or in the proper commercial swab transport device.
FRESH TISSUES - If shipping different tissue types, clearly label each container with owner and animal ID (if applicable) and sample/tissue type. Double bag ALL fresh tissues.
ORAL FLUID - Ensure container is securely sealed and labeled.

Additional Information

Includes PEDV, PDCoV, and TGE.