College of Veterinary Medicine

Influenza virus, Group A (matrix) PCR (Avian, Swine)

Section: Molecular Diagnostics

Fee: $40.00 ($40 for HPAI incident testing**)

Turnaround Time: 1 – 2 business days

Days Test Started: Monday, Tuesday, Wednesday, Thursday, Friday

Species: Avian, Canine, Chicken, Equine, Ovine, Porcine, Turkey

Specimen

Name

Container

Minimum Amount

Swabs (species appropriate)

Viral Transport Medium/Leakproof Tube

See Collection Information


Lung

Sealed, Leakproof Container

8 - 10 grams


Oral Fluid (porcine only)

Sealed Plastic Conical Tube

5 - 8 ml


Collection Information

For specifics on avian sample collection, please read the article from USDA Avian Sample Collection for Influenza A and Newcastle Disease. Please follow these guidelines and submit samples in the appropriate broth. Indicate the sample pooling method used on the submission form. To order BHI from NVSL, please visit NVSL Kit and Instrument Order Form.

SWAB (fecal, respiratory, tissue, purulent) - Synthetic (Dacron/polyester) swabs are recommended. Swabs should be placed in a viral transport system (examples: Viral Culturette, Becton Dickinson; Universal Transport Medium, Copan Diagnostics) or in a sterile, leakproof tube with 0.5 ml sterile saline. Please only utilize bacterial culturette systems for bacterial culture.
FRESH TISSUES - Collect tissues in sterile/clean containers (plastic jars or sealable plastic bags). Separate tissues in different containers and label with tissue type, animal ID, and owner name.
ORAL FLUID - Submit oral fluid in a leak proof container (i.e. plastic conical tube) with appropriate labeling.

Shipping Information

OVERNIGHT - Ship overnight in an insulated shipper with ice packs (pack absorptive material to collect moisture) DO NOT SHIP OVER THE WEEKEND OR HOLIDAY - NO SATURDAY DELIVERY
LEAKS - Ensure all samples are in leakproof packages and clearly labeled with Tissue Type/Animal ID/Owner Name. Include absorptive material to collect any leaks - double bag any fresh tissues.
PAPERWORK - Package paperwork separately in a waterproof, sealable plastic bag. Ensure paperwork is complete and specimens are labeled for easy matching. If you are using CF.755 Multiple Animal Continuation Form, please include a hard copy and email a copy to addl@purdue.edu.
BULK - Please include bulk material inside the shipping box to prevent samples from moving or being crushed during transit (loose glass blood tube and an ice pack will always break).

Additional Information
AVIAN
For increased avian mortalities where Avian Influenza is concerned, please contact the ADDL and Indiana Board of Animal Health as soon as possible and before submitting.
Avian Influenza samples testing positive on the matrix PCR will be further tested for H5 and H7. Positive samples for either H5 or H7 will be sent to NVSL for confirmation.

**Fee during HPAI incident - during an HPAI incident, samples will be charge at a rate of $40 per sample. There will be NO accessioning fee added to those cases to offset the pricing difference.
The Commercial Poultry Incident Form should be used when submitting samples related to a HPAI event.

SWINE
Swine Influenza (IAV-S) samples testing positive within the parameters for the USDA Surveillance Program will be further tested (Subtyping, Virus Isolation, Sequencing H & N) using the sample with the lowest CT value at no additional charge to the client. To qualify, Lung and Nasal Swab samples must have a CT value less than 25 OR an Oral Fluid sample with a CT value less than 20. For more information, see Appendix C for the Influenza A in Swine Testing Algorithm. Lung samples can only be tested individually and not pooled.

Purdue University College of Veterinary Medicine, 625 Harrison Street, West Lafayette, IN 47907, 765-494-7607

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